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ty1 tag  (New England Biolabs)


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    Structured Review

    New England Biolabs ty1 tag
    Ty1 Tag, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 9032 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ty1+tag/XhoI/pm40407324-382-38-45
    Average 99 stars, based on 9032 article reviews
    ty1 tag - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: TcCARP3 modulates compartmentalized cAMP signals involved in osmoregulation, infection of mammalian cells, and colonization of the triatomine vector in the human pathogen Trypanosoma cruzi
    Article Snippet: Briefly, Trypanosoma cruzi Y strain epimastigotes constitutively expressing T7 RNA polymerase and Cas9 were transfected with a sgRNA template obtained by PCR (primers 10 & 17; Table S2) and a donor DNA cassette, amplified from pMOTag23T vector (primers 11 & 12; Table S2). .. The pMOTag23T vector was made by amplifying two copies of the Ty1 tag from the pTREXh-2xTy1 vector (primer 13 & 14; Table S2) and cloned into pMOTag2T already containing a puromycin resistance marker and one copy of the Ty1 tag ( ) by XhoI site using NEBuilder® HiFi DNA Assembly cloning kit (New England Biolabs). ..

    Article Title: TcCARP3 modulates compartmentalized cAMP signals involved in osmoregulation, infection of mammalian cells, and colonization of the triatomine vector in the human pathogen Trypanosoma cruzi .
    Article Snippet: Briefly, T. cruzi Y strain epimastigotes constitutively expressing T7 RNA polymerase and Cas9 were transfected with a sgRNA template obtained by PCR (primers 10 and 17; Table S2) and a donor DNA cassette, amplified from pMOTag23T vector (primers 11 and 12; Table S2). .. The pMOTag23T vector was made by amplifying two copies of the Ty1 tag from the pTREXh-2xTy1 vector (primers 13 and 14; Table S2) and cloned into pMOTag2T already containing a puromycin resistance marker and one copy of the Ty1 tag (67) by XhoI site using NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs). ..

    Clone Assay:

    Article Title: TcCARP3 modulates compartmentalized cAMP signals involved in osmoregulation, infection of mammalian cells, and colonization of the triatomine vector in the human pathogen Trypanosoma cruzi
    Article Snippet: Briefly, Trypanosoma cruzi Y strain epimastigotes constitutively expressing T7 RNA polymerase and Cas9 were transfected with a sgRNA template obtained by PCR (primers 10 & 17; Table S2) and a donor DNA cassette, amplified from pMOTag23T vector (primers 11 & 12; Table S2). .. The pMOTag23T vector was made by amplifying two copies of the Ty1 tag from the pTREXh-2xTy1 vector (primer 13 & 14; Table S2) and cloned into pMOTag2T already containing a puromycin resistance marker and one copy of the Ty1 tag ( ) by XhoI site using NEBuilder® HiFi DNA Assembly cloning kit (New England Biolabs). ..

    Article Title: TcCARP3 modulates compartmentalized cAMP signals involved in osmoregulation, infection of mammalian cells, and colonization of the triatomine vector in the human pathogen Trypanosoma cruzi .
    Article Snippet: Briefly, T. cruzi Y strain epimastigotes constitutively expressing T7 RNA polymerase and Cas9 were transfected with a sgRNA template obtained by PCR (primers 10 and 17; Table S2) and a donor DNA cassette, amplified from pMOTag23T vector (primers 11 and 12; Table S2). .. The pMOTag23T vector was made by amplifying two copies of the Ty1 tag from the pTREXh-2xTy1 vector (primers 13 and 14; Table S2) and cloned into pMOTag2T already containing a puromycin resistance marker and one copy of the Ty1 tag (67) by XhoI site using NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs). ..

    Marker:

    Article Title: TcCARP3 modulates compartmentalized cAMP signals involved in osmoregulation, infection of mammalian cells, and colonization of the triatomine vector in the human pathogen Trypanosoma cruzi
    Article Snippet: Briefly, Trypanosoma cruzi Y strain epimastigotes constitutively expressing T7 RNA polymerase and Cas9 were transfected with a sgRNA template obtained by PCR (primers 10 & 17; Table S2) and a donor DNA cassette, amplified from pMOTag23T vector (primers 11 & 12; Table S2). .. The pMOTag23T vector was made by amplifying two copies of the Ty1 tag from the pTREXh-2xTy1 vector (primer 13 & 14; Table S2) and cloned into pMOTag2T already containing a puromycin resistance marker and one copy of the Ty1 tag ( ) by XhoI site using NEBuilder® HiFi DNA Assembly cloning kit (New England Biolabs). ..

    Article Title: TcCARP3 modulates compartmentalized cAMP signals involved in osmoregulation, infection of mammalian cells, and colonization of the triatomine vector in the human pathogen Trypanosoma cruzi .
    Article Snippet: Briefly, T. cruzi Y strain epimastigotes constitutively expressing T7 RNA polymerase and Cas9 were transfected with a sgRNA template obtained by PCR (primers 10 and 17; Table S2) and a donor DNA cassette, amplified from pMOTag23T vector (primers 11 and 12; Table S2). .. The pMOTag23T vector was made by amplifying two copies of the Ty1 tag from the pTREXh-2xTy1 vector (primers 13 and 14; Table S2) and cloned into pMOTag2T already containing a puromycin resistance marker and one copy of the Ty1 tag (67) by XhoI site using NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs). ..

    Cloning:

    Article Title: TcCARP3 modulates compartmentalized cAMP signals involved in osmoregulation, infection of mammalian cells, and colonization of the triatomine vector in the human pathogen Trypanosoma cruzi
    Article Snippet: Briefly, Trypanosoma cruzi Y strain epimastigotes constitutively expressing T7 RNA polymerase and Cas9 were transfected with a sgRNA template obtained by PCR (primers 10 & 17; Table S2) and a donor DNA cassette, amplified from pMOTag23T vector (primers 11 & 12; Table S2). .. The pMOTag23T vector was made by amplifying two copies of the Ty1 tag from the pTREXh-2xTy1 vector (primer 13 & 14; Table S2) and cloned into pMOTag2T already containing a puromycin resistance marker and one copy of the Ty1 tag ( ) by XhoI site using NEBuilder® HiFi DNA Assembly cloning kit (New England Biolabs). ..

    Article Title: TcCARP3 modulates compartmentalized cAMP signals involved in osmoregulation, infection of mammalian cells, and colonization of the triatomine vector in the human pathogen Trypanosoma cruzi .
    Article Snippet: Briefly, T. cruzi Y strain epimastigotes constitutively expressing T7 RNA polymerase and Cas9 were transfected with a sgRNA template obtained by PCR (primers 10 and 17; Table S2) and a donor DNA cassette, amplified from pMOTag23T vector (primers 11 and 12; Table S2). .. The pMOTag23T vector was made by amplifying two copies of the Ty1 tag from the pTREXh-2xTy1 vector (primers 13 and 14; Table S2) and cloned into pMOTag2T already containing a puromycin resistance marker and one copy of the Ty1 tag (67) by XhoI site using NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs). ..



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    Image Search Results


    KEY RESOURCES TABLE

    Journal: Developmental cell

    Article Title: Transcription factor NFYa controls cardiomyocyte metabolism and proliferation during mouse fetal heart development

    doi: 10.1016/j.devcel.2023.10.012

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: 1 μg/mL H3k27ac antibody (Diagenode, C15410196) or 4 μg/mL Ty1 tag antibody (Diagenode, C152000054) was added to each sample and incubated at 4°C overnight with gentle rotation.

    Techniques: Produced, Western Blot, Virus, Recombinant, Modification, Protease Inhibitor, Expressing, Software